Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet

Jun 29, 2016BMC veterinary research

Rumen bacterial toxins increase natural antimicrobial peptide in dairy cows' mammary glands on a high-concentrate diet

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Abstract

Lactating cows on a high-concentrate diet (HCD) showed higher levels of lipopolysaccharide (LPS) in ruminal fluid and blood compared to those on a low-concentrate diet (LCD).

  • Cows fed the HCD had a lower ruminal pH than those on the LCD.
  • LPS concentrations were increased in the rumen, lacteal artery, and vein in the HCD group.
  • Enhanced expression of antimicrobial peptides and pro-inflammatory cytokines was observed in the mammary glands of HCD-fed cows.
  • LAP protein levels were significantly higher in the HCD group compared to the LCD group.
  • While the expression of nuclear factor kappa B (NF-кB) remained unchanged, its activation increased in HCD-fed cows.

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