Full text is available at the source.
Involvement of second messengers in the signaling pathway of vitellogenesis-inhibiting hormone and their effects on vitellogenin mRNA expression in the whiteleg shrimp, Litopenaeus vannamei
Role of signaling molecules in how vitellogenesis-inhibiting hormone controls egg yolk protein gene activity in whiteleg shrimp
AI simplified
Abstract
Incubation with 100nM recombinant vitellogenesis-inhibiting hormone reduced vg mRNA expression to less than 20% of control levels after 8 hours.
- Intracellular cyclic guanosine monophosphate (cGMP) levels increased 3.2-fold after 2 hours of incubation with recombinant L. vannamei sinus gland peptide-G.
- cGMP levels reached 18-fold the control after 0.5 hours of incubation with recombinant peptide and a phosphodiesterase inhibitor.
- After 24 hours, vg mRNA expression decreased to less than 50% of control levels when exposed to 1μM A23187, a calcium ionophore.
- Both recombinant vitellogenesis-inhibiting hormone and calcium ionophore are associated with reduced vg mRNA expression in cultured ovary tissue.
- Increased intracellular cGMP and calcium may play a role in the signaling pathway affecting vg mRNA levels.
AI simplified