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Single pot organic solvent-free thermocycling technology for siRNA-ionizable LNPs: a proof-of-concept approach for alternative to microfluidics
Simple solvent-free heating method for making siRNA-delivery nanoparticles as an alternative to microfluidics
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Abstract
siRNA- were formulated with particle sizes of 104.2 ± 34.7 nm and an encapsulation efficiency of 83.3 ± 4.1%.
- A new may efficiently produce nucleic acid-ionizable LNPs without the need for organic solvents.
- Optimization of process parameters is necessary for precisely sized particles and effective nucleic acid encapsulation.
- Thermocycling siRNA-ionizable LNPs exhibited comparable morphological structures to those produced by commercial microfluidics ionizable LNPs.
- These thermocycling LNPs demonstrated a longer shelf life at 4°C compared to microfluidics-derived counterparts.
- The proposed method could address challenges such as high production costs and stability issues associated with traditional microfluidic technology.
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Key numbers
104.2 ± 34.7 nm
Particle Size
Size of thermocycling siRNA-.
83.3 ± 4.1%
Encapsulation Efficiency
Efficiency of siRNA encapsulation in thermocycling LNPs.
3 months
Shelf Life
Shelf life of thermocycling siRNA- at 4°C.