Tracking changes in dCREB2 during long-term memory formation in live fruit flies over time and brain areas

dCREB2 activity is persistently elevated in specific neurons of the ellipsoid body following long-term memory training.

• The Drosophila homologue of mammalian CREB, dCREB2, is associated with long-term memory formation.
• Elevated dCREB2 activity was found in the ellipsoid body R2/R4m neurons after memory training.
• In the mushroom body, dCREB2 activity was suppressed immediately after training and increased during later stages.
• Heterogeneous responses in multiple subsets of mushroom body neurons were observed during long-term memory processing.
• These findings indicate that dCREB2 plays a role in both the ellipsoid body and mushroom body during memory consolidation.

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