Highly sensitive detection of KRAS G12C mutation using a dual amplification system without PAM requirement

The method achieved a detection limit of 1.5 aM for the KRAS G12C mutation.

• A fluorescent biosensing platform was developed to detect a specific genetic mutation without the need for a PAM site.
• The platform utilizes CRISPR/Cas12a combined with a specific DNA amplification technique.
• Detection of KRAS G12C mutations was effective across a linear range from 10 aM to 10 pM.
• The system could identify mutations at a level as low as 0.1% in a sample concentration of 10 pM.
• It demonstrated strong performance in analyzing real biological samples.

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