TGF-β receptor 1 regulates progenitors that promote browning of white fat

Aug 14, 2018Molecular metabolism

TGF-β receptor 1 controls cells that help turn white fat into energy-burning brown fat

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Abstract

Deletion of TGF-β receptor 1 promotes beige adipogenesis while reducing the detrimental effects of a high-fat diet.

TGF-β signaling may regulate the appearance of beige adipocytes in white fat through interaction with the prostaglandin pathway.
Presumptive beige stem/progenitor cells can be isolated from the stromal vascular cells of white fat using flow cytometry techniques.
Genetic or pharmacologic inhibition of TGF-β signaling leads to high expression of beige markers in isolated progenitor cells.
Transplantation of TGF-β receptor 1-deficient stromal vascular cells into athymic nude mice results in robust beige adipogenesis when subjected to a high-fat diet and stimulation.
Controlled inhibition of TGF-β receptor 1 signaling, along with PGE2 stimulation, could promote beige adipogenesis and enhance metabolic functions.

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OBJECTIVE: Beige/brite adipose tissue displays morphological characteristics and beneficial metabolic traits of brown adipose tissue. Previously, we showed that TGF-β signaling regulates the browning of white adipose tissue. Here, we inquired whether TGF-β signals regulated presumptive beige progenitors in white fat and investigated the TGF-β regulated mechanisms involved in beige adipogenesis.

METHODS: We deleted TGF-β receptor 1 (TβRI) in adipose tissue (TβRImice) and, using flow-cytometry based assays, identified and isolated presumptive beige progenitors located in the stromal vascular cells of white fat. These cells were molecularly characterized to examine beige/brown marker expression and to investigate TGF-β dependent mechanisms. Further, the cells were transplanted into athymic nude mice to examine their adipogenesis potential. AdKO

RESULTS: Deletion of TβRI promotes beige adipogenesis while reducing the detrimental effects of high fat diet feeding. Interaction of TGF-β signaling with the prostaglandin pathway regulated the appearance of beige adipocytes in white fat. Using flow cytometry techniques and stromal vascular fraction from white fat, we isolated presumptive beige stem/progenitor cells (iBSCs). Upon genetic or pharmacologic inhibition of TGF-β signaling, these cells express high levels of predominantly beige markers. Transplantation of TβRI-deficient stromal vascular cells or iBSCs into athymic nude mice followed by high fat diet feeding and stimulation of β-adrenergic signaling via CL316,243 injection or cold exposure promoted robust beige adipogenesis in vivo.

CONCLUSIONS: TβRI signals target the prostaglandin network to regulate presumptive beige progenitors in white fat capable of developing into beige adipocytes with functional attributes. Controlled inhibition of TβRI signaling and concomitant PGE2 stimulation has the potential to promote beige adipogenesis and improve metabolism.

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TGF-β receptor 1 regulates progenitors that promote browning of white fat

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