Talanta

Improved Near-Infrared Light Sensor Combining Special Nanoparticles and Gene-Editing Tools to Measure Kanamycin Levels

Updated

Abstract

A detection limit of 0.284 nM for kanamycin was achieved using a novel biosensor.

  • The biosensor utilizes a CRISPR-Cas12a signal amplification mechanism for enhanced sensitivity.
  • A ZnO/CdS heterojunction photoanode increases photocurrent production by 96% compared to ZnO alone.
  • The sensor demonstrates a linear response range from 10 to 1000 nM for kanamycin detection.
  • Cross-reactivity with related compounds is less than 2.8%.
  • Average recovery rates in spiked milk samples are 97%, with a relative standard deviation of 2.2%.

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Funding

Competing interests

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
PubMed

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