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Development and validation of a simple UHPLC–MS/MS method for the simultaneous determination of trimethylamine N-oxide, choline, and betaine in human plasma and urine
Simple method to measure trimethylamine N-oxide, choline, and betaine in human blood and urine
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Abstract
A method was developed for the simultaneous determination of trimethylamine N-oxide, choline, and betaine in human plasma and urine with a total run time of 5 minutes.
- The method demonstrated excellent linearity for analytes in plasma (0.010-5.00μg/mL) and urine (1.00-150μg/mL).
- Intra- and inter-day accuracy and precision for all quality controls were within ±10%.
- High recovery rates were achieved for all analytes.
- The method was applied in a pilot study examining markers of atherosclerosis in kidney transplant patients.
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