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Simultaneous targeted analysis of trimethylamine-N-oxide, choline, betaine, and carnitine by high performance liquid chromatography tandem mass spectrometry
Measuring levels of trimethylamine-N-oxide, choline, betaine, and carnitine using advanced liquid chromatography and mass spectrometry
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Abstract
A sensitive method for detecting trimethylamine-N-oxide (TMAO) and related metabolites has been developed for mouse plasma analysis.
- TMAO is produced from dietary components such as choline, betaine, and carnitine through gut microbiota activity.
- Elevated levels of TMAO are linked to an increased risk of atherosclerosis and cardiovascular events.
- The new liquid chromatographic electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method allows for simultaneous measurement of TMAO, betaine, choline, and carnitine.
- The method has been validated for selectivity, sensitivity, precision, and accuracy in analyzing mouse plasma samples.
- Stability of the analytes under various conditions has been confirmed.
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