Biomedical chromatography : BMC

Measuring trimethylamine-N-oxide, L-carnitine, and γ-butyrobetaine levels in human blood plasma

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Abstract

An ultra-high-performance liquid chromatography-mass spectrometry method was validated for quantifying trimethylamine-N-oxide (TMAO) and related molecules in human blood plasma.

  • The method allows simultaneous measurement of TMAO, L-carnitine, and γ-butyrobetaine in plasma samples.
  • Quantification of TMAO was validated following US Food and Drug Administration guidelines.
  • The technique was successfully applied to plasma samples from healthy volunteers.
  • TMAO and its related molecules are of interest due to their potential implications in health.

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