Ultrastructural characteristics of circadian pacemaker neurones, immunoreactive to an antibody against a pigment-dispersing hormone in the fly's brain

May 26, 2004Neuroscience letters

Detailed structure of daily rhythm nerve cells marked by a pigment-dispersing hormone in the fly brain

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Abstract

PDH-ir varicosities in two fly species show accumulation of dense core vesicles (DCVs).

  • PDH-ir varicosities are identified as potential releasing sites for the PDH-like peptide within the neuronal circadian system.
  • Both Drosophila melanogaster and Musca domestica exhibit similar ultrastructural features in PDH-ir varicosities.
  • Post-embedding immuno-electron microscopy confirms the presence of PDH-like peptide within the dense core vesicles.
  • The lack of synaptic specializations at PDH-ir varicosities suggests a mechanism of non-synaptic paracrine release for the peptide.

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