WC-2 mediates WC-1-FRQ interaction within the PAS protein-linked circadian feedback loop of Neurospora

Quantification of protein interactions reveals that WC-2 is a key scaffold for the circadian clock in NEUROSPORA.

• WC-2 is a consistently present protein in the nucleus, while FRQ and WC-1 are expressed in a rhythmic manner.
• WC-2 is essential for the interaction between WC-1 and FRQ, as they do not interact without it.
• Estimates of protein quantities and complexes at various circadian times align with existing clock models.
• The interactions facilitated by WC-2 may contribute to the rhythmic expression of the central clock gene frequency (frq).

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