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Structural basis of AcrIF24 as an anti-CRISPR protein and transcriptional suppressor
How AcrIF24 works to block CRISPR and suppress gene activity
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Abstract
AcrIF24 binds to promoter DNA with nanomolar affinity.
- AcrIF24 is a homodimer that associates with two copies of the CRISPR surveillance complex (Csy).
- It inhibits the type I-F CRISPR-Cas system in Pseudomonas aeruginosa by preventing the hybridization of CRISPR RNA and target DNA.
- AcrIF24 also acts as an anti-CRISPR-associated (Aca) protein, repressing the transcription of the acrIF23-acrIF24 operon.
- The structure of the Csy-AcrIF24-promoter DNA complex at 2.7 Å reveals the mechanism for transcriptional suppression.
- AcrIF24 functions as a fusion protein combining both Acr and Aca activities, highlighting the diverse mechanisms of Acr proteins.
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