Altered Stra13 and Dec2 circadian gene expression in hypoxic cells

Overexpression of Stra13 and Dec2 is linked to altered circadian clock gene expression in response to hypoxia.

• Stra13 and Dec2, involved in cell growth and death, are overexpressed and lose their rhythmic patterns in fibroblasts exposed to CoCl2, simulating low oxygen conditions.
• This overexpression correlates with a loss of rhythmic activity in the clock genes Rev-erbalpha and Bmal1, as well as the clock-controlled gene Dbp.
• Cotransfection assays indicate that STRA13 and DEC2 can inhibit the activation of Rev-erbalpha and Dbp promoters by the CLOCK:BMAL1 complex.
• In a transplantable osteosarcoma tumor model, hypoxia is associated with disrupted circadian expression of Stra13, Dec2, Rev-erbalpha, Bmal1, and Dbp.

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