Locations where daily rhythm controllers BMAL1 and CLOCK bind within the mouse gene Rev-erbalpha

The mouse Rev-erbalpha gene contains at least two binding regions for the transcriptional activators BMAL1 and CLOCK.

• Two major feedback loops influence circadian rhythms in mammals.
• BMAL1/MOP3 and CLOCK activate circadian target genes by binding to specific DNA regions.
• The activity of BMAL1 and CLOCK is inhibited by PERIOD and CRYPTOCHROME proteins, affecting gene expression rhythmically.
• Rev-erbalpha acts as a repressor of Bmal1 and Clock genes within the second feedback loop.
• Not all potential binding sites for BMAL1 and CLOCK are occupied in living organisms, despite being identified in laboratory conditions.

Simplified