Fifty-one patients with Crohn's disease (CD) showed lower in their mucosa-associated microbiome compared to forty healthy controls.
Patients with CD exhibited distinct compared to healthy controls.
Twenty-three bacterial taxa were found to be differentially represented between CD patients and healthy controls.
Tyzzerella 4 was significantly overrepresented in patients with CD.
The microbiome in inflamed and proximal noninflamed ileal mucosa did not differ in alpha or beta diversity.
No bacterial taxa were differentially represented between inflamed and noninflamed mucosa within the same CD patients.
Simplified
BACKGROUND: Microbiota is most likely essential in the pathogenesis of Crohn's disease (CD). Fecal diversion after ileocecal resection (ICR) protects against CD recurrence, whereas infusion of fecal content triggers inflammation. After ICR, the majority of patients experience endoscopic recurrence in the neoterminal ileum, and the ileal microbiome is of particular interest. We have assessed the mucosa-associated microbiome in the inflamed and noninflamed ileum in patients with CD.
METHODS: Mucosa-associated microbiome was assessed by 16S rRNA sequencing of biopsies sampled 5 and 15 cm orally of the ileocecal valve or ileocolic anastomosis.
RESULTS: Fifty-one CD patients and forty healthy controls (HCs) were included in the study. Twenty CD patients had terminal ileitis, with endoscopic inflammation at 5 cm, normal mucosa at 15 cm, and no history of upper CD involvement. Crohn's disease patients (n = 51) had lower and separated clearly from HC on plots. Twenty-three bacterial taxa were differentially represented in CD patients vs HC; among these, Tyzzerella 4 was profoundly overrepresented in CD. The microbiome in the inflamed and proximal noninflamed ileal mucosa did not differ according to alpha diversity or beta diversity. Additionally, no bacterial taxa were differentially represented.
CONCLUSIONS: The microbiome is similar in the inflamed and proximal noninflamed ileal mucosa within the same patients. Our results support the concept of CD-specific microbiota alterations and demonstrate that neither ileal sublocation nor endoscopic inflammation influence the mucosa-associated microbiome.
Key numbers
2.4 × 10
Lower in CD Patients
measured in CD patients compared to healthy controls.
23
Differentially Represented Taxa
Number of bacterial taxa differentially represented in CD patients vs. healthy controls.
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