Circadian clock gene activity in gum and ligament cells is influenced by L-Mimosine and low oxygen in flat and 3D cultures

Human gingival fibroblasts and periodontal ligament fibroblasts express core circadian clock genes: Clock, Bmal1, Cry1, Cry2, Per1, Per2, and Per3.

• Expression of core circadian clock genes varies between monolayer and spheroid cultures of fibroblasts.
• The hypoxia mimetic agent L-Mimosine significantly reduced the expression of several clock genes in both types of fibroblasts.
• Hypoxia also decreased the levels of specific clock genes in fibroblasts, with variations noted between the cell types.
• The response to hypoxia and L-Mimosine suggests that these factors may influence circadian gene expression in periodontal cells.

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