cis-Regulatory Requirements for Tissue-Specific Programs of the Circadian Clock

CLK and CYC have distinct DNA targets in heads and bodies, revealing tissue-specific clock regulation.

• CLK and CYC bind to different sets of DNA in the head and body, indicating context-specific regulatory roles.
• Computational analysis identifies sequence motifs that suggest potential interactions with partner factors at binding sites.
• The opa motif is enriched in head binding sites, while the GATA motif is enriched in body binding sites.
• OPA and SERPENT (SRP) interact with CLK/CYC, possibly influencing their target selection in tissue-specific clock function.
• GATA motifs are essential for accurate gene expression in the adult fat body, midgut, and Malpighian tubules.

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