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Abstract
Detection limits of 1-100 copies µL⁻¹ were achieved within 20-45 minutes using CRISPR-Cas diagnostic protocols.
- Three field-ready assays were developed for detecting major plant pathogens: a lateral-flow test for DNA, a RT-RPA assay for RNA viruses, and an electrochemical biosensor.
- The assays provide analytical sensitivity comparable to conventional PCR diagnostics while significantly reducing assay time.
- Standardized workflows were created to address practical constraints such as sample inhibitors and reagent stability.
- The protocols facilitate rapid, on-site detection of plant diseases, enhancing plant health surveillance.
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