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Abstract
The DETECTR assay enables rapid detection of phytoplasma diseases using CRISPR/Cas12a technology combined with isothermal amplification.
- The assay amplifies target DNA from phytoplasmas using Recombinase Polymerase Amplification (RPA).
- Cas12a nuclease is activated to cleave both the amplified DNA and fluorescent oligonucleotide reporters after initial target cleavage.
- This process generates a fluorescent signal that indicates the presence of phytoplasma DNA.
- Guidelines are included for sample preparation, assay setup, and interpreting fluorescence data.
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