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Abstract
Effective guide RNA (gRNA) design is crucial for optimizing CRISPR/Cas9 gene editing outcomes.
- gRNA design rules can enhance on-target activity of Cas9 while reducing unintended off-target effects.
- A variety of computational tools are available to predict gRNA activity and assist in assay design.
- Understanding the balance between on-target and off-target activity is essential for successful gene editing.
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