Molecular cloning of a putative crustacean hyperglycemic hormone (CHH) isoform from extra-eyestalk tissue of the blue crab (Callinectes sapidus), and determination of temporal and spatial patterns of CHH gene expression

Aug 10, 2010General and comparative endocrinology

Cloning a sugar-regulating hormone gene from blue crab body tissue and tracking its activity over time and in different tissues

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Abstract

A cDNA encoding a putative crustacean hyperglycemic hormone isoform (CsCHH-2) was cloned from the thoracic ganglia of the blue crab.

  • CsCHH-2 is structurally similar to CsCHH-1, which was previously cloned from eyestalk ganglia.
  • Both isoforms have the same signal peptide and related peptide, but differ in their mature polypeptide structure.
  • CsCHH-1 is found exclusively in eyestalk neural ganglia, while CsCHH-2 is distributed across various tissues.
  • Transcript abundance of CsCHH-1 in eyestalk ganglia is significantly higher than that of CsCHH-2 in other tissues.
  • During the molt cycle, CsCHH-1 levels rise in eyestalk ganglia before molting, while CsCHH-2 levels peak earlier and drop before molting.

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