Molecular cloning of a cDNA encoding a crustacean hyperglycemic hormone from eyestalk ganglia of the blue crab, Callinectes sapidus

Apr 25, 2006General and comparative endocrinology

Cloning the gene for a blood sugar-regulating hormone from the eye nerve center of the blue crab

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Abstract

A cDNA (CsCHH-1) encoding a putative crustacean hyperglycemic hormone preprohormone was successfully cloned from blue crab (Callinectes sapidus) eyestalk ganglia.

  • The cloned CsCHH-1 includes structural features characteristic of crustacean hyperglycemic hormone preprohormones, such as a signal peptide and a C-terminal basic processing site.
  • The amino acid sequence of the mature CsCHH-1 polypeptide contains all signature domains associated with crustacean hyperglycemic hormones.
  • CsCHH-1 transcripts were exclusively found in eyestalk ganglia and were not detected in other tissues tested.
  • A similar CHH-like preprohormone transcript was identified in thoracic ganglion, ventral nerve cord, and brain, indicating potential tissue-specific expression patterns.

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