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Crustacean hyperglycemic hormone from the tropical land crab, Gecarcinus lateralis: Cloning, isoforms, and tissue expression
Cloning and tissue patterns of blood sugar-regulating hormone variants in the tropical land crab
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Abstract
Four distinct isoforms of crustacean hyperglycemic hormone (CHH) were identified, with differences in their genetic sequences.
- Isoforms EG-CHH-A, -B, -C, and -D were cloned from the eyestalk ganglia of the land crab Gecarcinus lateralis.
- All isoforms encode similar preprohormones, featuring a 28-amino acid signal peptide, a 42-amino acid precursor, and a 72-amino acid mature CHH.
- Each isoform contains characteristic amino acids, including six cysteines and two arginines, consistent with the neuropeptide family.
- A single amino acid difference exists between the mature form of EG-CHH-D and the other three isoforms.
- A fifth isoform, PO-CHH, was identified and shows a similar sequence to the CHH isoform from green crab, Carcinus maenas.
- The PO-CHH isoform differs in its sequence due to an insertion of a 111-base pair segment not found in the EG-CHH cDNAs.
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