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Cyclin B protein undergoes increased expression and nuclear relocation during oocyte meiotic maturation of the freshwater prawn Macrobrachium rosenbergii and the Chinese mitten crab Eriocheir sinensis
Increased levels and movement into the nucleus of Cyclin B protein during egg cell maturation in freshwater prawn and Chinese mitten crab
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Abstract
The prawn cyclin B cDNA encodes a 398 amino acid protein with a predicted molecular weight of 45.16 kDa.
- Immunodetection revealed a target band of approximately 53 kDa in the prawn ovaries during late vitellogenesis and germinal vesicle breakdown.
- A 41 kDa band was identified in the crab ovaries, indicating different protein processing between the species.
- Changes in cyclin B protein expression suggest that newly synthesized cyclin B proteins may be necessary for germinal vesicle breakdown in both prawn and crab.
- Immunohistochemical analysis showed that cyclin B proteins were localized in the ooplasm of previtellogenic oocytes and relocated to the germinal vesicle during vitellogenesis.
- Cyclin B proteins localized on the meiotic spindle during M phase, indicating a conserved role in meiotic processes in both species.
- Similar expression patterns of cyclin B proteins during oocyte maturation imply that the mechanisms for activating the M-phase promoting factor could be identical between prawn and crab.
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