Gene

Increased levels and movement into the nucleus of Cyclin B protein during egg cell maturation in freshwater prawn and Chinese mitten crab

Circadian Biology Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

The prawn cyclin B cDNA encodes a 398 amino acid protein with a predicted molecular weight of 45.16 kDa.

  • Immunodetection revealed a target band of approximately 53 kDa in the prawn ovaries during late vitellogenesis and germinal vesicle breakdown.
  • A 41 kDa band was identified in the crab ovaries, indicating different protein processing between the species.
  • Changes in cyclin B protein expression suggest that newly synthesized cyclin B proteins may be necessary for germinal vesicle breakdown in both prawn and crab.
  • Immunohistochemical analysis showed that cyclin B proteins were localized in the ooplasm of previtellogenic oocytes and relocated to the germinal vesicle during vitellogenesis.
  • Cyclin B proteins localized on the meiotic spindle during M phase, indicating a conserved role in meiotic processes in both species.
  • Similar expression patterns of cyclin B proteins during oocyte maturation imply that the mechanisms for activating the M-phase promoting factor could be identical between prawn and crab.

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Competing interests

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
PubMed

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