Molecular characterization and expression profiles of cdc2 and cyclin B during oogenesis and spermatogenesis in green mud crab (Scylla paramamosain)

The full-length cDNA of the Sp-cdc2 gene is 1593 bp, encoding a protein of 299 amino acids.

• Sp-cdc2 expression is significantly higher in the ovary compared to other tissues (P<0.01).
• No significant variation in Sp-cdc2 expression was observed across different stages of ovarian development (P>0.05), though T3 stage showed higher levels than T1 and T2 stages (P<0.05).
• The full-length cDNA of the Sp-cyclin B gene is 1492 bp, encoding a protein of 391 amino acids.
• Sp-cyclin B expression is highest in the ovary compared to other tissues (P<0.01) and significantly increases in the O5 stage compared to the previous four stages and the testis (P<0.05).
• In situ hybridization revealed that Sp-cdc2 and Sp-cyclin B transcripts are present in similar patterns during different stages of ovary and testis development.
• Both genes show higher expression levels in the oocytoplasm of certain oocyte stages and in specific spermatocyte stages.

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