Daily Variation of Clock Output Gene Activation in Behaviorally ArrhythmicmPer/mCryTriple Mutant Mice

None of the mPer/mCry triple mutant mice maintained circadian rhythmicity in constant darkness.

• Circadian rhythms in mammals are regulated by transcriptional feedback loops involving specific clock genes.
• The CLOCK/BMAL1 complex activates central clock genes such as mPer1, mPer2, mCry1, and mCry2.
• Triple mutant mice lacking mPer and mCry genes do not exhibit a 24-hour rhythm when deprived of light.
• Under light-dark conditions, some output genes in triple mutant mice continue to oscillate, suggesting partial rhythmic signaling.
• This highlights that a single mPer or mCry gene is insufficient to generate circadian rhythms independently.

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