Science (New York, N.Y.)

How the internal biological clock works in cultured skin cells

Circadian Biology Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

The peripheral oscillator in cultured fibroblasts shows identical characteristics to the oscillator in the suprachiasmatic nucleus (SCN).

  • Temporal expression profiles of all known clock genes are the same in both peripheral and SCN oscillators.
  • The phase of mRNA rhythms, including antiphase oscillation of Bmal1 and mPer genes, matches between the two oscillators.
  • There is a consistent delay between peak mRNA levels and the presence of nuclear mPER1 and mPER2 protein in both cell types.
  • Functional mCry genes are necessary to produce oscillations in both the peripheral and SCN oscillators.
  • The period length of oscillations is regulated by mCRY proteins in both systems.

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