Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Oct 18, 2018Reproduction (Cambridge, England)

Changes in fat-related gene activity during ovary growth in black tiger shrimp

AI simplified

Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

Microarray analysis revealed that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in the hepatopancreas and ovaries of black tiger shrimp.

Ovarian maturation in shrimp is linked to higher expression of specific genes involved in fatty acid metabolism in the hepatopancreas.
Fifteen fatty acid regulatory genes showed increased expression in the ovaries during ovarian maturation.
Polychaete feeding altered the expression of certain fatty acid-related genes, indicating a potential impact on shrimp physiology.
Eyestalk ablation led to increased expression of long-chain fatty acid-CoA ligase 4 in both the hepatopancreas and ovaries.
The study highlights the regulatory pathways involved in fatty acid dynamics during the ovarian development of crustaceans.

AI simplified

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaete Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes are yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after 1 month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

Full Text

Full text is available at the source.

View full text (HTML) ↗View full text (PDF) ↗View full text ↗

Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief