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Isolation and expression analysis of Bystin 1 transcript and protein during ovarian development of the giant tiger shrimp Penaeus monodon
Tracking Bystin 1 gene and protein during egg development in giant tiger shrimp
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Abstract
The full-length cDNA of bystin isoform 1 (PmBys1) in the giant tiger shrimp Penaeus monodon is 1553 bp long, encoding a polypeptide of 454 amino acids.
- PmBys1 mRNA levels are higher in ovaries than in other tissues of females and in testes of males in both juveniles and wild broodstock.
- In non-ablated wild female broodstock, PmBys1 mRNA progressively increases in ovaries from stage I to stage IV of development.
- Ovarian PmBys1 mRNA levels in stages I-IV are greater in eyestalk-ablated broodstock compared to non-ablated broodstock.
- Injection of exogenous serotonin increases ovarian PmBys1 mRNA significantly within 6-48 hours post injection.
- PmBys1 protein is present in ovarian stages I-V of non-ablated wild broodstock and stages II-IV of ablated wild broodstock.
- Localization of PmBys1 protein occurs in various compartments of oocytes during different stages of ovarian development.
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