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Molecular cloning and expression of progestin membrane receptor component 1 (Pgmrc1) of the giant tiger shrimp Penaeus monodon
Cloning and activity of a progesterone receptor in the giant tiger shrimp
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Abstract
The full-length cDNA of Pgmrc1 in giant tiger shrimp is 2015bp long and encodes a polypeptide of 190 amino acids.
- Pgmrc1 expression is greater in the ovaries of both intact and eyestalk-ablated broodstock compared to juveniles.
- Up-regulation of Pgmrc1 occurs earlier in the ovarian development of eyestalk-ablated shrimp, starting from the vitellogenic stage.
- In mature ovaries, Pgmrc1 expression is significantly higher in intact broodstock than in earlier stages.
- Pgmrc1 mRNA localizes in the cytoplasm of follicular cells and various stages of oocytes.
- Positive signals for Pgmrc1 protein are found in the follicular layers and cell membranes of follicular cells and oocytes.
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