Characterization and expression analysis of the Broad-complex (Br-c) gene of the giant tiger shrimp Penaeus monodon

Mar 15, 2013Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Analysis of the Broad-complex (Br-c) gene in the giant tiger shrimp

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Abstract

PmBr-c is 1897 bp in length and encodes a polypeptide of 442 amino acids.

PmBr-c is more abundantly expressed in the ovaries than in the testes of giant tiger shrimp.
Expression levels of PmBr-c mRNA in juvenile ovaries are significantly higher than in various stages of intact broodstock ovaries.
Significantly increased expression of PmBr-c occurs in stage III ovaries of wild broodstock and stage IV ovaries of eyestalk-ablated broodstock.
Ovarian PmBr-c expression is lower in 18-month-old domesticated shrimp compared to 6-month-old shrimp.
Injection of serotonin and progesterone significantly promotes PmBr-c expression in ovaries at specified times post-injection.
Treatment with 20-hydroxyecdysone significantly increases PmBr-c expression in juvenile shrimp ovaries.

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Broad-complex (Br-c) is the early ecdysone responsive gene encoding a family of zinc-finger transcription factors. In this study, the full-length cDNA of the Br-c gene of the giant tiger shrimp (Penaeus monodon) was identified. PmBr-c was 1897 bp in length containing an ORF of 1329 bp deducing to a polypeptide of 442 amino acids. PmBr-c was more abundantly expressed in ovaries than testes of P. monodon broodstock. The expression levels of PmBr-c mRNA in ovaries of juveniles was significantly greater than that in stages II (vitellogenic), IV (mature) and V (post-spawning) ovaries of intact broodstock. The expression level of PmBr-c was significantly increased in stage III (nearly mature) ovaries of intact wild broodstock and in stage IV ovaries of eyestalk-ablated broodstock (P<0.05). In domesticated broodstock, ovarian PmBr-c was expressed lower in 18-month-old shrimp compared to 6-month-old shrimp (P<0.05). In situ hybridization revealed that PmBr-c mRNA was localized in ooplasm of previtellogenic oocytes in various ovarian stages of P. monodon broodstock. Serotonin (5-HT, 50 μg/g body mass; 18-month-old shrimp) and progesterone (0.1 μg/g body mass; 14-month-old shrimp) injection significantly promoted the expression level of PmBr-c in ovaries of domesticated broodstock at 24 and 48-72 h post injection (hpi, P<0.05). The expression levels of PmBr-c in ovaries of juvenile P. monodon was significantly increased following 20-hydroxyecdysone treatment (1 μg/g body mass; 4-month-old shrimp) for 168 hpi (P<0.05). Taken together, PmBr-c seems to play an important role during ovarian development of P. monodon.

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Characterization and expression analysis of the Broad-complex (Br-c) gene of the giant tiger shrimp Penaeus monodon

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