Archives of toxicologyJan 18, 2005

Liver enzyme levels after alcohol exposure in mice lacking Aldh2

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Updated Mar 28, 2026

Abstract

CYP2E1, 2B1/2, and 4B1 enzyme levels increased by 2.61, 1.88, and 2.01 times in Aldh2 +/+ mice after ethanol exposure.

Ethanol exposure elevated CYP2E1, 2B1/2, and 4B1 protein levels in Aldh2 +/+ mice compared to their unexposed controls.
In Aldh2 -/- mice, CYP2E1, 2B1/2, and 4B1 expression levels were increased by 1.99, 1.05, and 1.33 times after ethanol treatment, respectively.
Aldh2 -/- mice exhibited significantly higher levels of CYP2E1 (4.19 times), 2B1/2 (2.89 times), and 4B1 (1.53 times) compared to Aldh2 +/+ mice without ethanol treatment.
These results indicate a potential role of CYP2E1, 2B1/2, and 4B1 in ethanol metabolism.
Individuals lacking Aldh2 may have higher levels of these enzymes compared to those with the Aldh2 gene.

Simplified

We determined expression levels of hepatic cytochrome P450 (CYP) 2E1, 2B1/2 and 4B1 enzymes in aldehyde dehydrogenase 2 (Aldh2) +/+ and Aldh2 -/- mice by immunoblotting assay following subchronic ethanol exposure for eight days. Using ethanol exposure, the protein expression levels of CYP2E1, 2B1/2 and 4B1 in Aldh2 +/+ mice were increased by factors of 2.61, 1.88 and 2.01 compared with Aldh2 +/+ mice that were not exposed to ethanol, respectively. On the other hand, in the Aldh2 -/- mice, CYP2E1, 2B1/2 and 4B1 protein expression levels after ethanol treatment were shown to be 1.99, 1.05 and 1.33 greater than those of Aldh2 -/- control mice, respectively. We also found an interesting fact in the present study; the Aldh2 -/- mice were shown to have higher CYP2E1 (by a factor of 4.19), 2B1/2 (by a factor of 2.89) and 4B1 (by a factor of 1.53) protein expression levels than Aldh2 +/+ mice despite the lack of ethanol treatment. These results suggest that CYP2E1, 2B1/2 and 4B1 play some role in ethanol metabolism and that Aldh2-deficient individuals may have higher levels of CYP2E1, 2B1/2 and 4B1 enzymes compared to Aldh2 wild-type individuals.

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Liver enzyme levels after alcohol exposure in mice lacking Aldh2

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