Ethanol‐Induced Oxidative DNA Damage and CYP2E1 Expression in Liver Tissue ofAldh2Knockout Mice

Acute ethanol exposure significantly increased hepatic 8-OHdG generation in Aldh2 +/+ mice but resulted in the highest levels in Aldh2 -/- mice.

• Oxidative DNA damage was assessed in Aldh2 +/+ and Aldh2 -/- mice after ethanol treatment.
• Olive tail moment measurements indicated no increase from ethanol exposure in either genotype.
• The Aldh2 genotype significantly influenced Olive tail moments after controlling for ethanol effects.
• The level of 8-OHdG, a marker of oxidative DNA damage, was notably elevated in Aldh2 -/- mice compared to Aldh2 +/+ mice.
• Increased 8-OHdG levels were associated with the hepatic expression of cytochrome P450 2E1 (CYP2E1).
• Findings suggest that individuals without ALDH2 may be more vulnerable to ethanol-related liver damage and cancer.

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