Mapping Aging Cells Showing p21 in Older Liver Tissue at Single-Cell Detail
Updated
Abstract
A fluorescence-guided approach identified diverse p21-positive senescent cell populations in aged liver.
- Senescent cells, which no longer divide, accumulate with age and are linked to various diseases.
- The method revealed a senescence-enriched cell population with identities including hepatocytes, macrophages, neutrophils, and plasma cells.
- Activated macrophages and hepatic stellate cells showed a higher likelihood of being in a senescent state compared to resting cells.
- Transcriptomic profiling of p21-expressing cells indicated varying senescence-associated secretory phenotype (SASP) programs across different cell types.
- An aggregation of cells expressing interferon-stimulated genes was identified, with limited overlap with p21-positive cells, indicating a separate aging-related stress response.
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Funding
Competing interests
J.H.L. is an inventor on U.S. Patent No. 12,319,955, filed by The Regents of the University of Michigan, which covers the Seq-Scope method for localized detection of nucleic acids in tissue samples. Y.H. is currently an employee of Samsung Semiconductor. All other authors declare no competing interests.
PubMed