Genetics and molecular biology

Identifying Genes Involved in Ovarian Development of the Giant Tiger Shrimp

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Abstract

A total of 452 expressed sequence tags (ESTs) were sequenced from the giant tiger shrimp ovaries.

  • Sequence assembly resulted in 28 contigs and 201 singletons, with 48.0% matching known sequences in GenBank.
  • Several transcripts related to reproduction were identified, including two full-length cDNAs for PmAPC11 and PmSePM.
  • Expression levels of PmSePM and keratinocyte-associated protein 2 decreased during ovarian development.
  • Chk1, mcm2, and egalitarian were down-regulated in the mature ovaries of wild P. monodon.
  • The expression profiles of PmSePM and keratinocyte-associated protein 2 may serve as biomarkers for reproductive maturation in domesticated P. monodon.

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