Light-Controlled CRISPR System Using Special RNA Structure for Accurate Single-Step Virus Testing

Detection sensitivity improved by 2 orders of magnitude, reaching a level of 1 copy/μL.

• A light-activated CRISPR/Cas12a system was developed to regulate activity through photoswitching.
• This system minimizes the risk of aerosol contamination during nucleic acid detection.
• Coupling with recombinase polymerase amplification enhances detection performance.
• The platform achieves sensitivity and specificity comparable to PCR for clinical samples.

Simplified