Journal of nanobiotechnology

Fast creation and improvement of special fats for effective mRNA delivery

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Updated

Abstract

A total of 623 alkyne-bearing were synthesized and assessed for mRNA delivery efficiency.

  • Ionizable lipids with 18-carbon alkyl chains, a cis-double bond, and ethanolamine head groups showed superior mRNA delivery capabilities.
  • Changes in the saturation, double bond placement, and chain length of ionizable lipids were linked to decreased mRNA delivery efficacy.
  • Alkynes located next to nitrogen atoms in ionizable lipids reduced the acid dissociation constant (pKa) of lipid nanoparticles, hindering mRNA delivery efficiency.
  • Transforming alkynes into alkanes significantly improved mRNA delivery both in laboratory settings and in living organisms.
  • Optimized ionizable lipids combined with cKK-E12 produced lipid nanoparticles that enhanced mRNA expression levels in living organisms.

Simplified

Key numbers

300×
Increase in mRNA delivery efficiency
Efficiency of mRNA delivery after reducing alkynes to alkanes.
623
Number of synthesized
Total lipids synthesized using the .

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Funding

Competing interests

The authors declare no competing interests.
PubMed

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