Keeping Mice at Comfortable Temperatures Changes Daily Gene Activity in Different Tissues

Thermoneutral housing reshaped tissue-specific circadian mRNA rhythms in mouse heart, liver, and diaphragm.

This RNA-seq mouse experiment sampled hearts, livers, and diaphragms every 4 hours over 48 hours in constant darkness, comparing room temperature at 25 C with thermoneutrality at 30 C.

The findings are tissue transcript rhythms in mice under controlled housing temperatures, not direct evidence of physiological or clinical outcomes.