Huang Lian Jie Du Decoction Prevents Chronic Alcoholic Encephalopathy and Improves Gut Microbiota Imbalance in Mice

Jun 24, 2025Molecular neurobiology

Herbal Treatment May Prevent Long-Term Alcohol Brain Damage and Improve Gut Bacteria Balance in Mice

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Abstract

(HLJDD) significantly improved voluntary motor and learning memory abilities in a mouse model of (CAE).

HLJDD treatment led to less damage in the prefrontal cortex and hippocampal CA1 of CAE mice.
Increased expression of synaptic proteins PSD95 and SYN was observed following HLJDD treatment (P < 0.01).
Analysis revealed that HLJDD altered the gut microbiota composition in CAE mice.
The findings suggest that HLJDD may have multiple beneficial effects on CAE, including anti-inflammatory and immunomodulatory actions.

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(HLJDD) has anti-inflammatory, immunomodulatory, intestinal flora regulation, and anticoagulant effects, but the effects and mechanisms of HLJDD on (CAE) remain unclear. This study aimed to investigate the effect and mechanism of HLJDD to improve CAE. CAE mouse model was established, and the mice were treated with different doses of HLJDD according to the principle of randomized controlled trial. Open field test and Morris water maze were used to observe the locomotor and learning memory abilities of mice, hematoxylin-eosin (HE) staining was used to observe the pathological changes in prefrontal cortex and hippocampal CA1, toluidine blue staining was used to detect the neuronal survival in prefrontal cortex and hippocampal CA1, transmission electron microscopy was used to examine neuronal micropathology in prefrontal cortex and hippocampal CA1, and immunofluorescent staining and western blot were used to detect the expression of postsynaptic density protein 95 (PSD95) and synaptophysin (SYN) in hippocampal CA1. Moreover, the changes in gut microbiota were analyzed by 16S rRNA sequencing. The present study found that HLJDD improved the voluntary motor ability and learning memory ability of CAE mice, attenuated prefrontal cortex and hippocampal CA1 damage, and increased the expression of synaptic remodeling-related proteins PSD95 and SYN (P < 0.01), and HLJDD treatment affected the composition of gut microbiota in mice with CAE. HLJDD is an effective traditional Chinese medicine against CAE, and regulating its gut microbiota imbalance.

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Huang Lian Jie Du Decoction Prevents Chronic Alcoholic Encephalopathy and Improves Gut Microbiota Imbalance in Mice

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