BACKGROUND: Activating autophagy promotes the invasion and progression of prostate cancer (PCa). Tetraspanin 1 () has been found to promote autophagy flux and its up-regulation can enhance the migration of PCa cells. In addition, there is a binding relationship betweenand the N6-methyladenosine (m6A) demethylase AlkB homolog 5 (). Therefore, we wanted to know whethercould affect autophagy by regulatingexpression, and thereby participate in PCa malignant progression. TSPAN1TSPAN1ALKBH5ALKBH5TSPAN1
METHODS: The expression ofandin PCa was examined by quantitative real-time polymerase chain reaction (qRT-PCR), and the functional tests included cell counting kit-8 and 5-ethynyl-2'-deoxyuridine (EdU) staining assays. The expression of autophagy-related proteins was confirmed by western blot. Detection of the m6A level ofwas performed using methylated RNA immunoprecipitation sequencing (MeRIP)-qPCR. ALKBH5TSPAN1TSPAN1
RESULTS: was significantly downregulated in PCa cells (LNCaP, DU145 and PC3 cells;< 0.001). Overexpression ofinhibited cell viability and the number of EdU-positive cells (< 0.01,< 0.001), decreased the ratio of microtubule-associated protein light chain 3B (LC3B)-II/LC3B-I, and promoted P62 protein expression in LNCaP and DU145 cells (< 0.001). The m6A level ofwas high in LNCaP and DU145 cells, but was inhibited by the overexpression of(< 0.001).overexpression promoted cell viability (< 0.001), increased EdU-positive cells and the LC3B-II/LC3B-I ratio (< 0.001,< 0.05), reduced P62 protein expression (< 0.05,< 0.001), and reversed the regulation ofoverexpression in LNCaP and DU145 cells (< 0.01,< 0.001). ALKBH5p ALKBH5p p p TSPAN1ALKBH5p TSPAN1p p p p p ALKBH5p p
CONCLUSIONS: Promotingexpression may inhibit PCa autophagy by reducing the m6A level of. ALKBH5TSPAN1
