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KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria
KaiA helps activate KaiC to keep the biological clock running in cyanobacteria
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Abstract
Activation of kaiBC expression by kaiA requires KaiC, indicating a positive feedback mechanism in the cyanobacterial circadian clock.
- KaiA is identified as a positive regulator of kaiBC expression.
- KaiC acts as a negative regulator in this autoregulatory system.
- Robust circadian phosphorylation of KaiC was observed.
- KaiA is essential for both in vivo phosphorylation of KaiC and in vitro autophosphorylation of KaiC.
- A long period mutation in kaiA2 attenuated the effects of KaiA on KaiC phosphorylation.
- A previously undocumented mutation in kaiC suppressed the long period phenotype and abnormal phosphorylation in the kaiA2 mutant.
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