Biosensors & bioelectronics

Light-activated CRISPR system with nano-amplifier for measuring non-DNA targets without amplification

Updated

Abstract

LANA achieved a detection limit of 50 pg/mL for cardiac troponin I (cTnI).

  • The platform utilizes a light-activated mechanism to enhance signal sensitivity and control activation timing.
  • It integrates immunomagnetic enrichment and aptamer-functionalized gold nanoparticles to improve detection performance.
  • LANA demonstrated a wide dynamic range of 0.05-500 ng/mL for quantifying cTnI.
  • This approach effectively reduces background activation, leading to more accurate readings.
  • The design is modular and can be adapted for detecting various protein or small-molecule biomarkers.

Simplified

Full Text

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Funding

Competing interests

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
PubMed

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