Analysis of mammalian circadian clock protein complexes over a circadian cycle

Discrete circadian complexes were identified with masses of 255 kDa for CLOCK-BMAL1 and 707 kDa for PER-CRY-CK1δ.

• Circadian rhythmicity is maintained by core clock proteins, including CLOCK, BMAL1, PER, CRY, and CK1δ.
• In mice, the expression of CLOCK and BMAL1 is regulated by the degradation of repressors in a daily cycle.
• Two distinct circadian complexes were characterized: one containing CLOCK-BMAL and another consisting of PER2, CRY1, and CK1δ.
• Data were obtained through centrifugation and chromatography of mouse liver extracts at various circadian times.
• Mass spectrometry analysis revealed the principal components of these circadian complexes are the core circadian proteins.

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