Myopia, or nearsightedness, is the most common form of refractive abnormality and is characterized by excessive ocular elongation in relation to ocular power. Retinal neurotransmitter signaling, including dopamine, is implicated in myopic ocular growth, but the visual pathways that initiate and sustain myopia remain unclear. Melanopsin-expressing retinal ganglion cells (mRGCs), which detect light, are important for visual function, and have connections with retinal dopamine cells. Here, we investigated how mRGCs influence normal and myopic refractive development using two mutant mouse models: Opn4mice that lack functional melanopsin photopigments and intrinsic mRGC responses but still receive other photoreceptor-mediated input to these cells; and Opn4mice that lack intrinsic and photoreceptor-mediated mRGC responses due to mRGC cell death. In mice with intact vision or form-deprivation, we measured refractive error, ocular properties including axial length and corneal curvature, and the levels of retinal dopamine and its primary metabolite, L-3,4-dihydroxyphenylalanine (DOPAC). Myopia was measured as a myopic shift, or the difference in refractive error between the form-deprived and contralateral eyes. We found that Opn4mice had altered normal refractive development compared to Opn4wildtype mice, starting ∼4D more myopic but developing ∼2D greater hyperopia by 16 weeks of age. Consistent with hyperopia at older ages, 16 week-old Opn4mice also had shorter eyes compared to Opn4mice (3.34 vs 3.42 mm). Opn4mice, however, were more hyperopic than both Opn4and Opn4mice across development ending with even shorter axial lengths. Despite these differences, both Opn4and Opn4mice had ∼2D greater myopic shifts in response to form-deprivation compared to Opn4mice. Furthermore, when vision was intact, dopamine and DOPAC levels were similar between Opn4and Opn4mice, but higher in Opn4mice, which differed with age. However, form-deprivation reduced retinal dopamine and DOAPC by ∼20% in Opn4compared to Opn4mice but did not affect retinal dopamine and DOPAC in Opn4mice. Lastly, systemically treating Opn4mice with the dopamine precursor L-DOPA reduced their form-deprivation myopia by half compared to non-treated mice. Collectively our findings show that disruption of retinal melanopsin signaling alters the rate and magnitude of normal refractive development, yields greater susceptibility to form-deprivation myopia, and changes dopamine signaling. Our results suggest that mRGCs participate in the eye's response to myopigenic stimuli, acting partly through dopaminergic mechanisms, and provide a potential therapeutic target underling myopia progression. We conclude that proper mRGC function is necessary for correct refractive development and protection from myopia progression. -/-DTA/DTA -/-+/+ -/-+/+ DTA/DTA +/+ -/--/-DTA/DTA +/+ -/-+/+ DTA/DTA -/-+/+ DTA/DTA -/-
