Methionine restriction prevents the progression of hepatic steatosis in leptin-deficient obese mice

Dietary methionine restriction (MR) reversed the severity of steatosis in the leptin-deficient ob/ob mouse.

• MR led to reduced hepatic triglyceride levels compared to control-fed mice.
• Despite similar food intake, body weight decreased in mice on the MR diet.
• Insulin and HOMA ratios were reduced, while levels of adiponectin increased in MR mice.
• Gene expression analysis showed downregulation of the Scd1 gene and upregulation of genes related to fatty acid oxidation.
• The VLDL secretion rate was significantly increased in MR mice, along with elevated mRNA levels of ApoB and Mttp.
• Inflammatory markers such as Tnf-α and Ccr2 were downregulated in response to MR.

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