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Rhythmic expression of miR‐27b‐3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver
Daily changes of miR-27b-3p control the clock gene Bmal1 after RNA is made in mouse liver
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Abstract
MiR-27b-3p exhibits rhythmic expression in the metabolic tissues of mice subjected to constant darkness.
- MiR-27b-3p's expression is induced in the livers of unfed and ob/ob mice.
- The oscillation phases of miR-27b-3p can be reversed by restricted feeding.
- Bioinformatics analysis indicates that Bmal1 may be a direct target of miR-27b-3p.
- Luciferase reporter assays demonstrate that miR-27b-3p suppresses Bmal1 activity in a dose-dependent manner.
- Overexpression of miR-27b-3p reduces the protein levels of BMAL1 and disrupts its rhythmicity.
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