Microbial biotechnology

Highly Specific One-Step Test with Visual Result for Detecting Monkeypox Virus Clade I

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated

Abstract

The one-pot detection assay for Monkeypox virus clade I can be completed within 40 minutes and has a limit of detection of 27.16 copies/μL.

  • MPXV clade I exhibits enhanced virulence and transmissibility, with a case fatality rate reaching 10%.
  • The assay demonstrates no cross-reactivity with MPXV clade II or other tested viruses, including Vaccinia virus.
  • Preliminary testing indicates the assay maintains detectable performance at room temperature (25°C).
  • Intra-assay and inter-assay repeatability are strong, with all coefficient of variation values below 10%.
  • The assay outperformed quantitative polymerase chain reaction in detecting low-concentration plasmid-spiked samples.

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