Pigment-dispersing factor and CCHamide1 in the Drosophila circadian clock network

Feb 14, 2023Chronobiology international

Roles of Pigment-Dispersing Factor and CCHamide1 in the Fruit Fly's Body Clock System

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Abstract

Approximately 98% of double mutants lacking specific neuropeptides are arrhythmic under constant dark conditions.

Single mutants displayed free-running rhythms in constant darkness, indicating some level of circadian function.
In light-dark conditions, double mutants showed phase-advanced evening activity compared to single mutants.
Both single and double mutants exhibited reduced morning activity, suggesting a shared impairment in morning rhythms.
The results indicate that interactions between specific neuropeptide signals influence behavioral rhythm parameters.
PDF signaling may suppress activity during the daytime in the absence of a functional circadian clock.

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Animals possess a circadian central clock in the brain, where circadian behavioural rhythms are generated. In the fruit fly (), the central clock comprises a network of approximately 150 clock neurons, which is important for the maintenance of a coherent and robust rhythm. Several neuropeptides involved in the network have been identified, including Pigment-dispersing factor (PDF) and CCHamide1 (CCHa1) neuropeptides. PDF signals bidirectionally to CCHa1-positive clock neurons; thus, the clock neuron groups expressing PDF and CCHa1 interact reciprocally. However, the role of these interactions in molecular and behavioural rhythms remains elusive. In this study, we generatedanddouble mutants and examined their locomotor activity-related rhythms. The single mutants ofordisplayed free-running rhythms under constant dark conditions, whereas approximately 98% of the double mutants were arrhythmic. In light-dark conditions, the evening activity of the double mutants was phase-advanced compared with that of the single mutants. In contrast, both the single and double mutants had diminished morning activity. These results suggest that the effects of the double mutation varied in behavioural parameters. The double and triple mutants of, andfurther revealed that PDF signalling plays a role in the suppression of activity during the daytime under a clock-less background. Our results provide insights into the interactions between PDF and CCHa1 signalling and their roles in activity rhythms. Drosophila melanogaster Pdf1CCHa1SK8Pdf1CCHa1SK8per, Pdf1 1CCHa1SK8

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Pigment-dispersing factor and CCHamide1 in the Drosophila circadian clock network

Abstract

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