Protocol for setup and circadian analysis of inverted feeding in mice

Aug 12, 2021STAR protocols

Method for scheduling and analyzing reversed feeding times in mice to study body clock effects

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Circadian BiologyCircadian Biology on OpenScience ā†—PubMed ā†—DOI ā†—

Abstract

Inverted feeding may provide a new method to study circadian clock synchronization in tissues.

  • Inverted feeding alters the feeding rhythm in mice, potentially impacting circadian rhythms.
  • Tissue dissection and RNA extraction are essential for analyzing gene expression related to circadian rhythmicity.
  • R software can be used to analyze circadian rhythmicity data derived from the tissue samples.
  • The CircaMetDB database may enhance mechanistic studies related to inverted feeding and circadian clocks.

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